| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wallace, Brianna C (10)
|
VASEVA |
54.00 |
42.10 |
-11.90 |
| 2 |
Zhang, Lari S (10)
|
VASEVA |
43.94 |
44.56 |
0.62 |
| 3 |
Baker, Sabrina L (10)
|
VATAC |
47.51 |
49.50 |
1.99 |
| 4 |
Swartzbaugh, Julia C (10)
|
VAODAC |
48.94 |
49.59 |
0.65 |
| 5 |
Britt, Lane M (10)
|
VATAC |
51.18 |
50.19 |
-0.99 |
| 6 |
Ayala-Irizarry, Alondra K (10)
|
VASEVA |
50.63 |
51.17 |
0.54 |
| 7 |
Barakey, Ryan K (10)
|
VAODAC |
48.54 |
51.41 |
2.87 |
| 8 |
Kohler, Olivia A (9)
|
VATAC |
53.96 |
51.83 |
-2.13 |
| 9 |
Fannin, Abigail R (10)
|
VASEVA |
49.77 |
51.89 |
2.12 |
| 10 |
McGowan, Elly G (9)
|
VASEVA |
1:01.03 |
54.95 |
-6.08 |
| 11 |
Shaltayeva, Victoria (9)
|
VASEVA |
58.49 |
58.70 |
0.21 |
| 12 |
Burke, Teagan G (10)
|
VASEVA |
1:16.00 |
1:01.03 |
-14.97 |
| 13 |
Kampmann, Greta H (10)
|
VAODAC |
1:00.00 |
1:11.11 |
11.11 |
| 14 |
Best, Abigail R (10)
|
VAODAC |
1:00.89 |
1:13.51 |
12.62 |
|
Owens, Jada S (9)
|
VASEVA |
51.61 |
NS |
- |
|
Wojcik, Melissa A (9)
|
VASEVA |
52.98 |
NS |
- |
|
Walters, Alanna E (9)
|
VAYOBX |
1:03.97 |
NS |
- |
|
Nunziata, Josie L (9)
|
VADIG |
50.40 |
NS |
- |